![Bioinformatics in Python; DNA Toolkit. Part 3: GC Content Calculation | by rebelCoder | Python in Plain English Bioinformatics in Python; DNA Toolkit. Part 3: GC Content Calculation | by rebelCoder | Python in Plain English](https://miro.medium.com/v2/resize:fit:1400/1*IG0j12g_g4b-OEukB7EkbA.png)
Bioinformatics in Python; DNA Toolkit. Part 3: GC Content Calculation | by rebelCoder | Python in Plain English
![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![Bioinformatics in Python; DNA Toolkit. Part 3: GC Content Calculation | by rebelCoder | Python in Plain English Bioinformatics in Python; DNA Toolkit. Part 3: GC Content Calculation | by rebelCoder | Python in Plain English](https://miro.medium.com/v2/resize:fit:1400/1*pEy_gx3FiivFy5RvC8QDpg.png)